The invention relates to wastewater treatment application technologies and aims to provide a method for decolorizing printing and dyeing wastewater by adopting co-immobilized white-rot fungi. The method comprises the following steps: inoculating a seed solution containing any two or more white-rot fungi into a liquid culture medium with an immobilization carrier at a volume ratio of 5-20%, and then performing shake culture at 25-30 DEG C for 5-7 days; after covering the immobilization carrier with thalli, performing separation to obtain a carrier fixed with the white-rot fungus thalli, and putting the carrier into the printing and dyeing wastewater, thereby realizing decolorization treatment. The method fully utilizes enzymes generated by the white-rot fungi with different species and complementary enzyme systems to degrade and decolorize the printing and dyeing wastewater, can realize complete degradation of printing and dyeing wastewater pollutants, and can realize repeated decolorization. The method can be used for decolorization treatment of the printing and dyeing wastewater containing dyes with different structure types, and is high in decolorization efficiency. As the pollutants are degraded by the white-rot fungi, degradation products do not cause secondary pollution to a water body.